ccs -j 10 /public1/home/t0s000552/rna-seq/iso-seq/whfs-xs-240004_PB/xz1/1.rawdata/bam/m64033_240606_094212.subreads.bam  ./movie.ccs.bam --report-file movie.ccs.report --min-rq 0.9 
lima movie.ccs.bam primers.fasta movieX.fl.bam --isoseq --peek-guess
samtools merge movie.merged.bam movieX.fl.primer_5p--primer_3p.bam
isoseq3 refine movieX.fl.primer_5p--primer_3p.bam primers.fasta movieX.flnc.bam --require-polya --num-threads 10
isoseq3 cluster movieX.flnc.bam clustered.bam
pbmm2 align clustered.bam ../hic-final-green.fasta.masked.fasta aligned.sorted.bam
samtools sort  aligned.sorted.bam -o aligned.sorted_sor.bam
isoseq3 collapse aligned.sorted_sor.bam movie.ccs.bam out.gff --do-not-collapse-extra-5exons --log-level INFO