DATASET_HEATMAP
#In heatmaps, each ID is associated to multiple numeric values, which are displayed as a set of colored boxes defined by a color gradient
#lines starting with a hash are comments and ignored during parsing
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# MANDATORY SETTINGS #
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#select the separator which is used to delimit the data below (TAB,SPACE or COMMA).This separator must be used throughout this file.
#SEPARATOR TAB
SEPARATOR SPACE
#SEPARATOR COMMA
#label is used in the legend table (can be changed later)
DATASET_LABEL example_heatmap
#dataset color (can be changed later)
COLOR #ff0000
#define labels for each individual field column
FIELD_LABELS GT PL CE GH AA CBM
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# OPTIONAL SETTINGS #
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#Heatmaps can have an optional Newick formatted tree assigned. Its leaf IDs must exactly match the dataset FIELD_LABELS.
#The tree will be used to sort the dataset fields, and will be displayed above the dataset. It can have branch lengths defined.
#All newlines and spaces should be stripped from the tree, and COMMA cannot be used as the dataset separator if a FIELD_TREE is provided.
FIELD_TREE (((f1:0.2,f5:0.5):1,(f2:0.2,f3:0.3):1.2):0.5,(f4:0.1,f6:0.5):0.8):1;
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# all other optional settings can be set or changed later #
# in the web interface (under 'Datasets' tab) #
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#Each dataset can have a legend, which is defined using LEGEND_XXX fields below
#For each row in the legend, there should be one shape, color and label.
#Optionally, you can define an exact legend position using LEGEND_POSITION_X and LEGEND_POSITION_Y. To use automatic legend positioning, do NOT define these values
#Optionally, shape scaling can be present (LEGEND_SHAPE_SCALES). For each shape, you can define a scaling factor between 0 and 1.
#To order legend entries horizontally instead of vertically, set LEGEND_HORIZONTAL to 1
#Shape should be a number between 1 and 6, or any protein domain shape definition.
#1: square
#2: circle
#3: star
#4: right pointing triangle
#5: left pointing triangle
#6: checkmark
#LEGEND_TITLE,Dataset legend
#LEGEND_SCALE,1
#LEGEND_POSITION_X,100
#LEGEND_POSITION_Y,100
#LEGEND_HORIZONTAL,0
#LEGEND_SHAPES,1,2,3
#LEGEND_COLORS,#ff0000,#00ff00,#0000ff
#LEGEND_LABELS,value1,value2,value3
#LEGEND_SHAPE_SCALES,1,1,0.5
#left margin, used to increase/decrease the spacing to the next dataset. Can be negative, causing datasets to overlap.
#MARGIN 0
#width of the individual boxes
#STRIP_WIDTH 25
#always show internal values; if set, values associated to internal nodes will be displayed even if these nodes are not collapsed. It could cause overlapping in the dataset display.
#SHOW_INTERNAL 0
#show dashed lines between leaf labels and the dataset
#DASHED_LINES 1
#if a FIELD_TREE is present, it can be hidden by setting this option to 0
#SHOW_TREE 1
#define the color for the NULL values in the dataset. Use the letter X in the data to define the NULL values
#COLOR_NAN #000000
#automatically create and display a legend based on the color gradients and values defined below
#AUTO_LEGEND 1
#define the heatmap gradient colors. Values in the dataset will be mapped onto the corresponding color gradient.
COLOR_MIN #FFFFFF
COLOR_MAX #FF0000
#you can specify a gradient with three colors (e.g red to yellow to green) by setting 'USE_MID_COLOR' to 1, and specifying the midpoint color
#USE_MID_COLOR 1
COLOR_MID #FFC0CB
#By default, color gradients will be calculated based on dataset values. You can force different values to use in the calculation by setting the values below:
USER_MIN_VALUE 0
USER_MID_VALUE 150
USER_MAX_VALUE 300
#border width; if set above 0, a border of specified width (in pixels) will be drawn around individual cells
#BORDER_WIDTH 0
#border color; used only when BORDER_WIDTH is above 0
#BORDER_COLOR #0000ff
#display or hide the text labels above each field column
#SHOW_LABELS 1
#text label size factor
#SIZE_FACTOR 1
#text label rotation
#LABEL_ROTATION 0
#text label shift in pixels (positive or negative)
#LABEL_SHIFT 0
#align fields labels to the tree circle; only applies in circular display mode and when FIELD_TREE is not displayed
#LABEL_ALIGN_TO_TREE,0
#normalize the data using the function specified in NORMALIZATION_TYPE. DATA_NORMALIZATION can be set to
#'rows' (normalize data for each tree leaf) or 'columns' (normalize data for each heatmap field)
#DATA_NORMALIZATION rows
#type of normalization to apply. The following types are available:
#normal: values will be normalized between values A and B (specified in NORMALIZATION_RANGE) using the formula A+(val-min)*(B-A)/(max-min)
#mean: values will be normalized between -1 and 1 using the formula (val-mean)/(max-min)
#scaling: values will be Z-score normalized using the formula (val-mean)/std.dev
#NORMALIZATION_TYPE scaling
#used to define the A-B range for standard normalization
#NORMALIZATION_RANGE 0 3
#Display the actual values inside the heatmap boxes. Can be either 'original' or 'normalized'. If the data is not normalized, original values will be used in any case.
#DISPLAY_VALUES normalized
#round the display values to this number of decimal points
#ROUND_VALUES 2
#if set to 1, display values using scientific notation (e.g. 1e3 is 1000). Can be combined with ROUND_VALUES
#VALUES_SCI_NOTATION 1
#color of the value labels
#VALUE_COLOR #0000ff
#if set to 1, calculate the value label color automatically, based on the darkness of the heatmap box. Labels will be white or black.
#VALUE_AUTO_COLOR 1
#shift the position of the value labels by the specified number of pixels (X and Y)
#VALUE_SHIFT 0 0
#rotate the value labels by this angle
#VALUE_ROTATION 0
#multiply the value label font size by this factor
#VALUE_SIZE_FACTOR 1
#Internal tree nodes can be specified using IDs directly, or using the 'last common ancestor' method described in iTOL help pages
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# Actual data follows after the "DATA" keyword #
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DATA
'Gymnopilus_junonius<7>_30' 162 12 40 239 87 63
'Hypholoma_sublateritium<1>*_12' 154 15 51 252 118 70
'Lentinula_edodes<9>*_0' 148 12 34 274 86 76
'Pholiota_conissans<5>*_1' 156 14 45 267 123 78
'Psilocybe_subaeruginosa<6>*_40' 179 12 54 252 131 89
'Rhodocollybia_butyracea<8>*_18' 169 13 40 287 99 66
'Schizophyllum_commune<17>*_0' 152 23 42 280 69 69
'Stropharia_rugosoannulata<0>*_49' 169 12 56 277 141 80
'Hygrocybe_coccinea<3>*_36' 166 8 25 174 59 50
'Hygrocybe_punicea<2>_16' 69 2 9 79 10 19
'Porpolomopsis_calyptriformis<12>*_2' 122 5 13 146 25 26
'Hygrophorus_russula<10>*_79' 102 6 14 141 38 19
'Hygrophorus_pudorinus<11>_33' 67 2 8 88 18 11
'Lichenomphalia_hudsoniana<22>_13' 205 8 31 232 53 59
需要修改的地方： 1、FIELD_LABELS，样本名称，以空格为分隔符
2、DATA ，叶名称为行名，列数应该与样本名称数目相对应